I-Gel Yellow Blood Collection Tube

Ijeli yokwahlula iityhubhu zokuqokelela igazi ziye zasetyenziswa ngokubanzi kwiilebhu zeklinikhi.Ukwahlula ijeli kunokwenza umaleko wokuzihlukanisa phakathi kwamacandelo eseli kunye ne-serum (i-plasma), ukuthintela ngokufanelekileyo ukutshintshiselana kwezinto phakathi kweeseli zegazi kunye ne-serum (i-plasma), kunye nokuqinisekisa ukuzinza kwe-serum (i-plasma) yamacandelo ngaphakathi kwexesha elithile.Ukwahlula iglu ikakhulu yenziwe irabha abicah, hydrocarbons macromolecular, iglu hydrophobic, etc. Njengesixhobo sepolymer, ayinyibiliki emanzini kunye ne-inert.I-thixotropic viscous liquid kunye nobuninzi be-1.04-1.05 mmol / Phakathi kwe-L, ineenzuzo zokuxhathisa i-oxidation, ukumelana nobushushu obuphezulu, ukumelana nobushushu obuphantsi, kunye nokuqina komoya okulungileyo.Ubuninzi be-serum ngu-1.026-1.031 mmol / L, kunye ne-hematocrit yi-1.090-1.095.Ngenxa yobunzima obuthile, i-gel yokwahlula iphakathi nje kwe-serum kunye neeseli zegazi, ngoko ke phantsi kweemeko eziqhelekileyo, igazi liya kubonakala ngokulandelelana emva kwe-centrifugation.ISerum, ijeli yokwahlula, kunye neeseli zegazi 3 imigangatho.

Kukho ngokubanzi iindidi ezimbini zokwahlula ijeli yetyhubhu yokuqokelela igazi edla ngokusetyenziswa kwiilabhoratri: ityhubhu yokwahlula ijeli ye-serum kunye ne-plasma yokwahlula ityhubhu ye-anticoagulation.I-serum yokwahlula i-gel procoagulation ityhubhu kukongeza i-coagulant kwityhubhu yokuqokelela igazi ukuze unciphise ixesha lokuhlangana kwegazi, ufumane i-serum ngokukhawuleza, kwaye uxele iziphumo ngelona xesha lifutshane.Iityhubhu zokuqokelela igazi leglasi akufuneki ukuba zongeze i-coagulants, kwaye igazi elidibana nodonga lwetyhubhu yeglasi liya kubangela ukuhlangana.Nangona kunjalo, xa izinto zokudibanisa i-XI kunye ne-XII zidibana neetyhubhu zokuqokelela igazi zeplastiki, amandla abo okusebenza abuthathaka kakhulu, kwaye i-coagulant kufuneka ifakwe ukunciphisa ixesha lokudibanisa.I-plasma yokwahlula i-gel anticoagulation ityhubhu itshizwe nge-anticoagulants efana ne-lithium heparin kudonga lwangaphakathi lwe-gel yokwahlula ityhubhu yokuqokelela igazi ukuhlangabezana neemfuno zovavanyo olungxamisekileyo lwe-plasma ye-biochemical.

Kwizicelo ezisebenzayo, kuhlala kubonwa ukuba isiphumo sokwahlula ityhubhu yokuqokelela igazi yejeli ayilungile, umzekelo: kwezinye iityhubhu zerabha zokwahlula, kunokubonwa ukuba amaqhekeza ejeli okanye amathontsi e-oyile adada phezu komhlaba. i-serum okanye imisiwe kwi-serum;umaleko wejeli wokwahlula udada kumaleko weserum.ngasentla, njl njl. Ukwahlula iijeli nako kunokuphazamisana nezinye iziphumo zovavanyo.Kwisebe lethu, kuye kwafunyaniswa ukuba i-batch ethile ye-reagents kunye ne-serum yokwahlula i-gel ekhawulezayo ityhubhu isabelana nomnye ngexesha lokufunyanwa kwe-HBSAg yenkqubo yokufumanisa i-Abbott i2000SR, okukhokelela kwiziphumo ezilungileyo zobuxoki.

Eli phepha lihlalutya ngokukodwa kwiinkalo ezimbini, oko kukuthi, izizathu zempembelelo embi yokwahlula i-gel yokwahlula, kunye nefuthe lokungeniswa kwejeli yokwahlula kumlinganiselo.

1. Indlela yokwahlula i-serum kunye ne-plasma ngokwahlula i-gel Ukwahlula i-gel yi-thixotropic mucocolloid ehlanganiswe ne-hydrophobic organic compounds kunye ne-silica powder.Isakhiwo siqulethe inani elikhulu leebhondi ze-hydrogen.Ubukho beebhondi ze-hydrogen zenza isiseko sekhemikhali ye-thixotropy ye-gel yokwahlula..Ubunzima obuthile be-gel yokwahlula bugcinwa kwi-1.05, ubunzima obuthile becandelo legazi legazi li malunga ne-1.02, kunye nobukhulu obuthile becandelo legazi elibunjwe malunga ne-1.08.Xa ijeli yokwahlula kunye negazi elidibeneyo (okanye i-anticoagulated yegazi elipheleleyo) i-centrifuged kwi-tube yokuvavanya efanayo, Ngenxa yamandla e-centrifugal esetyenziswe kwijeli yokwahlula, isakhiwo sothungelwano lwe-hydrogen bond saphulwa kwisakhiwo esifana nekhonkco, kunye nokwahlula. ijeli iba yi-low-viscosity fluid.Ngenxa yobunzima obuthile obuhlukeneyo, ijeli yokwahlula iguqulwa iguqulwe kwaye ihlulwe ukuze yenze iileyi ezintathu zegazi legazi (igazi elichasene ne-anticoagulated) / ijeli yokwahlukana / i-serum (i-plasma).Xa i-centrifuge iyeka ukujikeleza kwaye ilahlekelwa amandla e-centrifugal, amasuntswana ekhonkco kwijeli yokwahlula abumba isakhiwo sothungelwano kwakhona ngamabhondi e-hydrogen, abuyisele ijeli ye-high-viscosity ephezulu, kwaye enze umaleko wokuzihlukanisa phakathi kwe-serum (plasma) kunye nehlwili legazi (anticoagulated). igazi elipheleleyo)..

2. Izizathu zempembelelo embi yokwahlula ijeli yokwahlula

2.1 Ukwahlula uMgangatho weGel Ubunzima obuthile bejeli yokwahlula phakathi kwe-serum (i-plasma) kunye neeseli zegazi, eyona siseko somzimba sokubuyisela ukuguqulwa kwejeli yokwahlula kunye nokuhlukana kwe-serum (i-plasma).Ukuba umgangatho wejeli yokwahlula kwityhubhu yokuqokelela igazi ihlwempuzekileyo kwaye ubunzima obuthile abuhambelani neemfuno, ngokuqinisekileyo kuya kuchaphazela umphumo wokwahlula i-serum (i-plasma), kunye nesiganeko sokuba i-gel yokwahlula kunye ne-serum (i-plasma) ezithungelanayo zinokwenzeka.

2.2 Ukudibanisa kwegazi okungaphelelanga Emva kwe-centrifugation, ngamanye amaxesha i-gel compartment yokwahlula kunye ne-serum kunye ne-blood clots ayihlukanga ngokupheleleyo, kwaye i-fibrin filaments ibonakala kwi-serum.Isizathu sisoloko kukuba igazi aliqini ngokupheleleyo phambi kwe-centrifugation.I-coagulation yegazi engaphelelanga inokubangela ukuba i-fibrin ixutywe kwindawo yodwa.Ityhubhu yerabha yokwahlula i-serum kufuneka isetyenziswe ngokuchanekileyo ngokwemiyalelo, kwaye i-serum inokulungiswa nge-centrifugation emva kokuba igazi lidibene ngokupheleleyo (ngokubanzi, ityhubhu yeplastiki equlethe i-coagulant kufuneka ibekwe nkqo malunga nemizuzu engama-30, kunye negazi. ityhubhu yokuqokelela ngaphandle kwe-coagulant kufuneka ibekwe ngokuthe tye kwi-60-90 imizuzu).iisampulu zeserum ezikumgangatho ophezulu.

2.3 Ukushisa kwe-Centrifugation Ukushisa kwe-centrifugation kunempembelelo ebalulekileyo kwimpembelelo yokwahlula i-serum kwi-tube ye-gel yokwahlukana.I-serum yayicacile kwi-inert yokwahlula ijeli ityhubhu ye-coagulation ekhawulezayo eyahlulwe yi-centrifuge eqhelekileyo kwiqondo lobushushu begumbi, kodwa amaso anamafutha anobukhulu obahlukeneyo avela kwi-15% ukuya kwi-20% yeesampuli.Kwelinye icala, akukho amaso okunamafutha afunyenwe kwi-serum eyahlulwe kwityhubhu yokuvavanya i-centrifuged yi-centrifuge yobushushu obuphantsi.Xa iqondo lokushisa lidlula iqondo lokushisa elifunekayo kwijeli yokwahlukana, i-gel inert iya kuchithwa kwi-serum.Ayizukuthintela kuphela kwaye ingcolise inaliti yesampulu kunye nekomityi yokuphendula ye-analyzer ye-biochemical, kodwa kwakhona ibe nempembelelo enkulu kwiziphumo zomlinganiselo we-biochemical.