Iityhubhu ze-PRP ze-Acd
Inkcazelo emfutshane:
I-Anticoagulant Citrate Dextrose Solution, eyaziwa ngokuba yi-ACD-A okanye i-Solution A iyisisombululo esingenayo i-pyrogenic, esiyinyumba.Esi sixhobo sisetyenziswa njenge-anticoagulant ekuveliseni i-plasma ene-platelet-rich (PRP) kunye ne-PRP Systems yokucubungula igazi elingaphandle.
I-Anticoagulant Citrate Dextrose Solution, eyaziwa ngokuba yi-ACD-A okanye i-Solution A iyisisombululo esingenayo i-pyrogenic, esiyinyumba.Esi sixhobo sisetyenziswa njenge-anticoagulant ekuveliseni i-plasma ene-platelet-rich (PRP) kunye ne-PRP Systems yokucubungula igazi elingaphandle.I-Anticoagulants esekelwe kwi-citrate isebenzisa amandla e-citrate ion kwi-chelate ionized calcium ekhoyo egazini ukukhusela ukudibanisa kwegazi kunye nokwenza i-non-ionized calcium-citrate complex.
Ukuphela kwemveliso ye-anticoagulant ukuba i-United States yokutya kunye noLawulo lweDrugs luvunyiwe ukuba lusetyenziswe ukulungiselela i-PRP kwiiNkqubo ze-PRP ezahlukeneyo yi-ACD-A.Ngokophando olwenziwa ngo-2016 kwi-PRP efunyenwe ngee-anticoagulants ezahlukeneyo kunye nemiphumo yazo ekuziphatheni kweeseli ze-mesenchymal stromal in vitro kunye neenombolo zeplatelet, kukho iziphumo ezithandekayo ekusebenziseni i-PRP ukulungiswa kwezicubu ze-musculoskeletal.
Ukwahlula iiplatelet kucetyiswa ukuba kuthathelwe indawo i-citrate ye-sodium esemgangathweni ye-Acid Citrate Dextrose (ACD-A) njengoko inkqubo yokuzimela ifuna amanyathelo amaninzi okuhlamba.IiPlatelets zizinzile ngakumbi kwi-37C ngexesha lokusonta, kodwa ukuzijikeleza kubushushu begumbi (25 C) nako kusebenza kakuhle.Ukunciphisa i-pH nge-ACD-A (isondela kwi-6.5) inceda ukuphazamisa ukusebenza kwe-thrombin eshiyekileyo kwiityhubhu zeplatelet, kwaye igalelo kugcino olupheleleyo lwe-platelet morphology ngelixa utshintshela umsebenzi kwi-minima.Ngokwesiqhelo kufuneka uphinde umise iiplatelet kwiTyrode Buffer eyiyo (pH 7.4) ukuze ubuyisele ukusebenza kwazo.I-ACD ineenzuzo ezininzi xa kufikwa ekugcineni iiplatelet
Xa i-ACD isetyenziswe, iziphumo zabonisa isivuno esiphezulu seplatelet kwigazi elipheleleyo.Nangona kunjalo, ukusetyenziswa kwe-EDTA kwakhuthaza ukukhula kwi-platelet volume mean emva kokuba amanyathelo e-centrifugation yegazi aqhutywe ukufumana i-PRP.Emva koko, ukusetyenziswa kwe-ACD kubangele ukwanda kweeseli ze-mesenchymal stromal.Ngoko ke, kwagqitywa ukuba i-anticoagulants equka i-ACD-A idlala indima enkulu ekulungiseleleni i-PRP kwaye inceda ukunyusa inkqubo ukuya kwinqanaba elikhulu.
