Akukho-Additive Blood Collection Umbhobho oBomvu
Inkcazelo emfutshane:
Ukufunyanwa kwe-biochemical, i-immunological experiments, i-serology, njl.
Ukusetyenziswa kwe-inhibitor yokubambelela kwigazi ekhethekileyo kusombulula ngokufanelekileyo ingxaki yokunamathela kwigazi kunye nokuxhoma eludongeni, ukuqinisekisa isimo sokuqala segazi ukuya kwinqanaba eliphezulu kunye nokwenza iziphumo zovavanyo zichaneke ngakumbi.
Imvelaphi: Abantu abane-hyperglycemia, ngakumbi abo bakhulelweyo, baxhomekeke kwimilinganiselo echanekileyo yeswekile yegazi ukuze baxilongwe ngokuchanekileyo isifo seswekile kunye nokubeka iliso.I-Glycolysis emva kokutsalwa kwegazi, nangona kunjalo, iyancipha ukuxinana kweglucose egazini eliqokelelwa kwiqondo lobushushu begumbi ngokungabikho kozinzo.Ubushushu obubandayo (4°C) buthintela i-glycolysis;kodwa ukupholisa ngokukhawuleza kunye nokulungiswa kwesampulu yegazi nganye kubanda kunzima ukufezekisa kwiinkqubo zesiqhelo zeklinikhi.Ngoko ke, izilondolozo zisetyenziselwa ukuzinzisa i-glucose ngexesha lokuqokelela igazi kunye neenkqubo zokucubungula ezenziwa kwiqondo lokushisa.Olu phononongo luvavanye isiphumo se-anticoagulants ezahlukeneyo (EDTA, i-heparin, i-oxalate), kunye okanye ngaphandle kwe-glycolysis inhibitors (NaF, citrate), ekuzinzeni kweglucose kwiisampulu zeplasma - efunyenwe kwigazi eliqokelelwe kwaye ligcinwe kwiqondo lobushushu begumbi ukuya kuthi ga ngoku. 24h.
Iindlela: Igazi le-venous liqokelelwe kumavolontiya angama-60;isampulu nganye yegazi yomxhasi yahlulahlulwe yaba yimibhobho emithandathu, nganye iqulethe ukwakheka okwahlukileyo kwe-anti-glycolysis-anticoagulant.I-Terumo VENOSAFE™ Iityhubhu zeGlycemia eziqulethe i-NaF/citrate buffer)/Na2EDTA;I-NaF/Na-heparin;kunye ne-NaF/K2oxalate.Iityhubhu ze-Sarstedt ziqulethe i-NaF / citrate;I-NaF/Na2EDTA;kunye ne-K2EDTA.Kwi-0, i-2, i-8 kunye ne-24 h, i-plasma yafunyanwa kwimilinganiselo ye-glucose usebenzisa i-Glucose Hexokinase kunye neendlela ze-Glucose Oxidase, kunye ne-ADVIA® 1800 Clinical Chemistry System.
Iziphumo: Zombini iindlela zibonise i-glycolysis encinci nge-24 h (<3.8%) kwii-tubes ezintathu zeTerumo VENOSAFE™ Glycemia, kunye ne-Sarstedt S-Monovette GlucoEXACT ityhubhu equlethe i-NaF / citrate.I-Glycolysis yayiphezulu kwiibhubhu eziqukethe i-NaF / Na2EDTA-yodwa (11.7%) kunye ne-K2EDTA-yodwa (85%).
Izigqibo: Iityhubhu ze-Glycemia (eziqulethe i-NaF/citrate buffer/Na2EDTA; i-NaF/Na-heparin; kunye ne-NaF/K2oxalate) kunye ne-Sarstedt S-Monovette® GlucoEXACT imibhobho (equlethe i-NaF/citrate) ifanelekile ukuhambisa iisampulu zegazi elipheleleyo kwi-venous ukuya kuvavanyo. ilabhoratri ngaphakathi kwe-24 h kwiqondo lokushisa.
