Ityhubhu emhlophe yokufunyanwa kwe-Nucleic Acid
Inkcazelo emfutshane:
Isetyenziselwa ngokukodwa ukufumanisa i-nucleic acid, kwaye iveliswa ngokupheleleyo phantsi kweemeko zokuhlanjululwa, okunciphisa ungcoliseko olunokwenzeka ngexesha lenkqubo yokuvelisa kunye nokunciphisa ngokufanelekileyo impembelelo yokungcola okunokwenzeka kwimifuniselo.
1. Uvavanyo lomthamo wokufunxa: Umthamo wokufunxa, oko kukuthi, ubungakanani begazi elitsalwayo, unempazamo ngaphakathi kwe-±10%, kungenjalo awufaneleki.Umlinganiselo ongachanekanga wegazi elitsalwayo yeyona ngxaki inkulu ngoku.Oku akuphumeleli kuphela kwiziphumo zokuhlola ezingachanekanga, kodwa kubangela ukuvala kunye nomonakalo kwisixhobo sokuhlola.
2. Umfuniselo wokuvuza kwesikhongozeli: Ityhubhu yokuqokelela igazi efunxayo equlethe isisombululo se-sodium fluorescein composite yabekwa ijongise phezulu emanzini adiyoniweyo kangangemizuzu engama-60.Ngaphantsi komthombo wokukhanya we-ultraviolet wexesha elide, akukho fluorescence yabonwa phantsi kombono oqhelekileyo kwigumbi elimnyama, elalifanelekile.Ukuvuza kwesikhongozeli sesona sizathu siphambili somthamo wegazi ongachanekanga wetyhubhu yangoku yokuqokelela igazi.
3. Uvavanyo lwamandla e-Container: i-container iphantsi kwe-centrifuge kunye ne-centrifugal acceleration ye-3000g kwimizuzu eyi-10, kwaye ifanelekile ukuba ayiphuli.Iimfuno ezingqongqo kumazwe angaphandle zezi: 2 yeemitha ngaphezu komhlaba, ityhubhu yokuqokelela igazi ye-vacuum iwela ngokuthe nkqo ngaphandle kokuphuka, enokuthintela umonakalo owenziwe ngengozi kwityhubhu yokuvavanya kunye nokulahlekelwa kwemizekelo.
4. Ubuncinci bovavanyo lwesithuba esisimahla: Ubuncinci besithuba sokuqinisekisa ukuba igazi lixutywe ngokupheleleyo.Ubungakanani begazi elitsalwayo ngu-0.5ml-5ml, > + 25% yexabiso legazi elitsalweyo;ukuba umlinganiselo wegazi elitsalwayo ngu>5ml,>15% wegazi elitsaliweyo.
5. Uvavanyo oluchanekileyo lwe-solvent, i-solute mass ratio kunye nexabiso lokongeza isisombululo: impazamo kufuneka ibe phakathi kwe-± 10% yesityalo esiqhelekileyo esichaziweyo.Le yingxaki ngokulula kwaye ixhaphakile, kwaye sesinye sezizathu eziphambili zedatha yovavanyo olungachanekanga.
