Ulwazi lweMveliso
Intsingiselo yophuhliso lweshishini kunye neendaba zamva nje
Ekuqaleni kweminyaka yoo-1940, itekhnoloji yokuqokelela igazi yevacuum yaqanjwa, eyashiya amanyathelo angeyomfuneko anjengokuzoba ityhubhu yenaliti kunye nokutyhala igazi kwityhubhu yovavanyo, kwaye kwasetyenziswa ityhubhu yokufunxa igazi ezenzekelayo eyenziwe ngaphambili kwityhubhu yokufunxa ukunciphisa ukuba nokwenzeka kwe-hemolysis. ubukhulu obukhulu.Ezinye iinkampani zezixhobo zonyango nazo zazise ezazo iimveliso zokufunxa igazi, kwaye ngeminyaka yoo-1980, kwaqaliswa isigqubuthelo setyhubhu entsha yokhuseleko.Isigqubuthelo sokhuseleko siquka isigqubuthelo seplastiki esikhethekileyo esigqume ityhubhu yokufunxa kunye neplagi yerabha esandula ukuyilwa.Ukudibanisa kunciphisa amathuba okuqhagamshelana neziqulatho zombhobho kwaye kuthintele ukudibanisa ngomnwe kunye negazi eliseleyo phezulu nasekupheleni kweplagi.Le ngqokelela ye-vacuum ene-cap yokhuseleko inciphisa kakhulu umngcipheko wokungcoliswa ngabasebenzi bezempilo ukusuka ekuqokeleleni ukuya ekuqhutyweni kwegazi.Ngenxa yezinto ezicocekileyo, ezikhuselekileyo, ezilula kunye nezithembekileyo ze-istics, inkqubo yokuqokelela igazi isetyenziswe ngokubanzi kwihlabathi kwaye iye yanconywa yi-NCCLS njengesixhobo esiqhelekileyo sokuqokelela igazi.Ukuqokelelwa kwegazi ngevacuum kwasetyenziswa kwezinye izibhedlele zase China phakathi kwiminyaka yoo-1990.Okwangoku, ukuqokelelwa kwegazi kwevacuum kuye kwamkelwa ngokubanzi kwizibhedlele ezininzi kwizixeko ezinkulu neziphakathi.Njengendlela entsha yokuqokelelwa kwegazi leklinikhi kunye nokufumanisa, umqokeleli wegazi wevacuum yinguqu yokuqokelela igazi lemveli kunye nokugcinwa.
Isikhokelo sokuSebenza
Inkqubo yokuQokelelwa koMfanekiso
1. Khetha iibhubhu ezifanelekileyo kunye nenaliti yokuqokelela igazi (okanye isethi yokuqokelela igazi).
2. Gcotha ngobunono iityhubhu ezinezongezo zokukhupha nayiphi na into enokuthi ibambelele kwisithinteli.
3. Sebenzisa i-tourniquet kwaye uhlambulule indawo ye-venipuncture kunye ne-antiseptic efanelekileyo.
4. Qinisekisa ukuba ubeka ingalo yesigulana kwindawo esezantsi.
5. Susa isiciko senaliti uze wenze i-venipuncture.
6. Xa igazi livela, gqobhoza isithinteli serubha setyhubhu kwaye ukhulule i-tourniquet ngokukhawuleza.Igazi liya kumpompoza kwityhubhu ngokuzenzekelayo.
7. Xa ityhubhu yokuqala igcwele (igazi liyeka ukugeleza kwi-tube), susa ngobunono ityhubhu kwaye utshintshe ityhubhu entsha.(Jonga kuMyalelo oCetyisiweyo wedro)
8. Xa ityhubhu yokugqibela igcwele, susa inaliti kwi-vein.Sebenzisa iswabhu eyomileyo ecocekileyo ukucofa indawo yokugqobhoza kude kuphele ukopha.
9. Ukuba ityhubhu iqulethe i-additive, guqula ngobumnene ityhubhu ngamaxesha angama-5-8 ngokukhawuleza emva kokuqokelela igazi ukuqinisekisa umxube owaneleyo wokudibanisa kunye negazi.
10. I-tube engekho-additive kufuneka ibe yi-centrifuged ngaphambi kwemizuzu engama-60-90 emva kokuqokelela igazi.I-tube iqulethe i-activator ye-clot kufuneka ibe yi-centrifuged ngaphambi kwemizuzu eyi-15-30 emva kokuqokelela igazi.Isantya se-centrifugal kufuneka sibe yi-3500-4500 rpm / min (i-relative centrifugal force> 1600gn) kwi-6-10 imizuzu.
11. Uvavanyo lwegazi lonke kufuneka lwenziwe kungadlulanga iiyure ezi-4.Isampuli yeplasma kunye neserum esahluliwe kufuneka ihlolwe ngaphandle kokulibazisa emva kokuqokelela.Lo mzekelo kufuneka ugcinwe kwiqondo lokushisa elichaziweyo ukuba uvavanyo alukwazi ukwenziwa ngexesha.
Izinto Eziyimfuneko Kodwa Azibonelelwanga
Iinaliti zokuqokelela igazi kunye nezibambi (okanye iiseti zokuqokelela igazi)
Tourniquet
I-swab yotywala
Ulungelelwaniso olucetyisiweyo lweDro
ityhubhu eluhlaza okwesibhakabhaka, ityhubhu ebomvu, ityhubhu ye-orange, ityhubhu ephuzi, ityhubhu eluhlaza, ityhubhu yelavender, ityhubhu emnyama, ityhubhu engwevu
Phawula:Kunconywa ukusebenzisa ityhubhu yokulahla (ityhubhu ebomvu) njengetyhubhu yokuqala eza kutsalwa ukuba isethi yokuqokelela igazi isetyenziswa.Oko kuya kuqinisekisa umlinganiselo ofanelekileyo we-anticoagulant-to-blood.
Izilumkiso kunye neZilumkiso
1. Ukusetyenziswa kwe-in vitro kuphela.
2. Musa ukusebenzisa imibhobho emva komhla wokuphelelwa.
3. Musa ukusebenzisa iityhubhu ukuba iityhubhu zophuke.
4. Kuphela ukusetyenziswa kanye.
5. Musa ukusebenzisa imibhobho ukuba kukho into yangaphandle.
6. Iityhubhu ezinophawu STERILE zifakwe inzala kusetyenziswa i-Co60.
7. Imiyalelo mayilandelwe ngokuchanekileyo ukuqinisekisa ukusebenza kakuhle.
8. I-tube iqulethe i-clot activator kufuneka ibe yi-centrifuged emva kokuhlanganiswa kwegazi ngokupheleleyo.
9. Kuphephe ukuchanabeka kwiityhubhu kwilanga elithe ngqo.
10.Nxiba iiglavu ngexesha lokupuntshulwa ukuze unciphise ingozi yokuvezwa
Ugcino
Gcina iibhubhu kwi-18-30 ° C, umswakama we-40-65% kwaye ugweme ukutshatyalaliswa kwelanga.Musa ukusebenzisa iityhubhu emva komhla wokuphelelwa kwawo oboniswe kwiilebhile.