I-Muclear Cell ye-Gel ye-Separation Tube enye-CPT Tube

I-Muclear Cell ye-Gel yoLwahlulo ityhubhu enye—I-CPT yeTube eFakelwe umfanekiso

Inkcazelo emfutshane:

Isetyenziselwa ukwahlula i-monocytes kwigazi elipheleleyo.

Isetyenziswa kakhulu ekubhaqweni kokusebenza komzimba we-lymphocyte okufana ne-HLA, ukufumanisa i-leukemia eshiyekileyo yofuzo kunye nonyango lwe-immune cell.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Yintoni na I-CPT Tube

I-tube eyodwa ye-muclear cell gel (ityhubhu ye-CPT) yongezwa kunye ne-hypaque, i-anticoagulant kunye ne-gel yokwahlukana.I-Lymphocytes kunye neeseli ze-mononuclear zinokuhlukaniswa ngokulula kwigazi elipheleleyo nge-centrifugation enenqanaba elinye xa usebenzisa ijeli ekhethekileyo yokwahlula iiseli.Isetyenziselwa ikakhulu ukufumanisa i-lymphocyte immune function, i-HLA okanye intsalela ye-leukemia yofuzo yofuzo kunye nonyango lwe-immune cell cell.Inika indlela esemgangathweni yokutsalwa kwenyathelo elinye le-monocytes ukulungiselela isampula yokuxilonga yeklinikhi kunye ne-immunotherapy yeselula.

Umsebenzi weMveliso

1) Ubungakanani: 13 * 100mm, 16 * 125mm;

2) uMqulu owongezelelweyo: 0.1ml, 135usp;

3) Umthamo wegazi: 4ml, 8ml;

4) Ubomi beShelf: iinyanga ezingama-24 ukususela kumhla wokwenziwa;

5) Ugcino:Gcina kwi-18-25℃.

ImvelisoInzuzo

1) Esebenzayo, echanekileyo kwaye ekhuselekileyo;

I-2) Nge-Ficoll Hypaque eyakhelwe ngaphakathi, i-anticoagulant kunye ne-gel yokwahlula, iiseli ze-mononuclear zihlukaniswe kwigazi elipheleleyo nge-centrifugation-step-step.

3) Itekhnoloji echanekileyo yokwahlula iiseli.

4) Udonga lwangaphakathi lwe-bionic membrane processing technology;

I-5) Izinga lokubuyisela i-monocytes lingaphezulu kwe-90%, ubunyulu bungaphezulu kwe-95%, kwaye izinga lokusinda lingaphezulu kwe-99%

Imibandela Efuna Ingqalelo

Ezi ngongoma zilandelayo kufuneka ziqatshelwe:

1) Xa usenza uvavanyo lweeseli zokulima, nikela ingqalelo ekusebenzeni kwe-aseptic, inzalo ii-reagents (isisombululo sokwahlula, isisombululo sokuhlamba, njl.) kunye nezixhobo.Lo msebenzi kufuneka uqhutywe ziingcali ukuqinisekisa ukuhambelana komsebenzi.

2) Ubushushu beCentrifugation buqhelekile kubushushu begumbi (2~25℃).

3) Ngokuqhelekileyo xa ukwahlula iiseli ze-mononuclear (PBMC) kunye neFicoll, ubuninzi begazi elibomvu lincinci, elinokuthi lichaphazele ukuchaneka kovavanyo.Ngoko i-lysate ingasetyenziselwa (ezinye kufuneka zihlanjululwe), lawula ixesha le-lysis kwaye uthintele ukuchaphazela umsebenzi weeseli ze-mononuclear.

4) Lumka malunga nokuphinda kufakwe i-centrifugation enokuphinda kabini ukuhlanjululwa.

Ngaphambili: I-CTAD yokuFumana iThubhu

Okulandelayo: Umbhobho weGazi oBomvu