I-Platelet-rich plasma (PRP) yi-autologous concentration yeeplatelet zabantu kwiplasma.Ngokusebenzisa i-degranulation ye-alpha granules kwiiplatelet, i-PRP inokugcina izinto ezahlukeneyo zokukhula, kubandakanywa i-platelet-derived growth factor (PDGF), i-vascular endothelial growth factor (VEGF), i-fibroblast growth factor (FGF), i-hepatocyte growth factor (HGF), kunye nokuguqula ukukhula kwezinto (TGF), eziye zabhalwa ukuba ziqalise ukuphulukiswa kwesilonda kunye nokukhuthaza ukwanda kunye nokuguqulwa kweeseli ze-endothelial kunye ne-pericytes zibe zihluma ze-endothelial.
Iindima ze-PRP kunyango lokukhula kweenwele ziye zaxelwa kuphando oluninzi lwakutshanje.Uebel et al.baye bafumanisa ukuba izinto zokukhula kweplatelet plasma zonyusa isivuno seeyunithi zefollicular kuqhaqho lwempandla yamadoda.Umsebenzi wakutshanje ubonise ukuba i-PRP ikwandisa ukwanda kweeseli ze-papilla ze-dermal kwaye ibangele utshintsho olukhawulezayo lwe-telogen-to-anagen usebenzisa imodeli ye-vivo kunye ne-vitro.Olunye uphando lubonise ukuba i-PRP ikhuthaza ukubuyiswa kweenwele kunye nokunciphisa kakhulu ixesha lokubunjwa kweenwele.
Zomibini i-PRP kunye ne-platelet-poor plasma (PPP) ibandakanya ukuncediswa ngokupheleleyo kweeprotheni ze-coagulation.Kwisifundo esikhoyo, impembelelo ye-PRP kunye ne-PPP ekukhuleni kweenwele kwi-C57BL / 6 iigundane zaphandwa.I-hypothesis yayiyeyokuba i-PRP ibe nefuthe elihle ekukhuleni kobude beenwele kunye nokunyuka kwenani leenwele zeenwele.
Izilwanyana zovavanyo
Iyonke i-50 esempilweni ye-C57BL/6 yeempuku zamadoda (iiveki ezi-6 ubudala, i-20 ± 2 g) zafunyanwa kwiZiko leZilwanyana zeLabhoratri, iYunivesithi yaseHangzhou Eqhelekileyo (i-Hangzhou, eChina).Izilwanyana zazityiswa ukutya okufanayo kwaye zigcinwe kwindawo engaguqukiyo phantsi komjikelo we-12:12-h wokukhanya nobumnyama.Emva kweveki ye-1 ye-acclimatization, iigundane zahlulwe ngokungahleliwe zibe ngamaqela amathathu: iqela le-PRP (n = 10), iqela le-PPP (n = 10), kunye neqela lokulawula (n = 10).
Iprothokholi yophononongo yamkelwa yikomiti yemigaqo yeziko yophando lwezilwanyana phantsi koMthetho woPhando lweZilwanyana kunye neMimiselo esemthethweni eTshayina.
Ukulinganisa ubude beenwele
Kwi-8, i-13, kunye neentsuku ze-18 emva kwesitofu sokugqibela, iinwele ze-10 kwimouse nganye zikhethwe ngokungaqhelekanga kwindawo ekujoliswe kuyo.Imilinganiselo yobude beenwele yenziwa kwiinkalo ezintathu kusetyenziswa i-electron microscope, kwaye i-avareji yazo ibonakaliswe njengeemilimitha.Iinwele ezinde okanye ezonakeleyo zazingabandakanywa.
Hematoxylin kunye eosin (HE) staining
Iisampulu zesikhumba se-dorsal zakhutshwa kwiintsuku ze-18 emva kwesitofu sesithathu.Emva koko iisampuli zalungiswa kwi-10% engathathi hlangothi ye-formalin, ifakwe kwiparafini, kwaye isikwe kwi-4 μm.Amacandelo abhakwa i-4 h ukwenzela i-deparaffinization kwi-65 ° C, ifakwe kwi-ethanol ye-gradient, kwaye ifakwe kwi-hematoxylin ye-5 min.Emva kokuhlukana kwi-1% ye-hydrochloric acid alcohol, amacandelo afakwe emanzini e-ammonia, ahlanjwe nge-eosin, kwaye ahlanjululwe ngamanzi adibeneyo.Ekugqibeleni, amacandelo aphelelwe ngamanzi kunye ne-ethanol ye-gradient, ehlanjululwe nge-xylene, efakwe kwi-resin engathathi hlangothi, kwaye yabonwa ngokusebenzisa i-microscopy ekhanyayo (i-Olympus, i-Tokyo, i-Japan).
Ixesha lokuposa: Oct-12-2022